. 2022 Mar 21;12(14):8668–8673. doi: 10.1039/d2ra00087c

Comparison of the kinetic parameters of HRP depending on MNDH and H₂O₂ concentrations, including Michaelis–Menten constants (K_m) and maximum reaction rates (V_max) at pH 4.0 and 7.0.

Enzyme	pH	Substrate	K _m (μM)	V _max (ΔF s⁻¹)
HRP	4.0	H₂O₂	18.14	0.38
	4.0	MNDH	0.66	7.11
	7.0	H₂O₂	313.19	0.08
	7.0	MNDH	952.70	0.20

Comparison of the kinetic parameters of HRP depending on MNDH and H2O2 concentrations, including Michaelis–Menten constants (Km) and maximum reaction rates (Vmax) at pH 4.0 and 7.0.