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. Author manuscript; available in PMC: 2022 Nov 14.
Published in final edited form as: ACS Nano. 2021 Aug 11;15(8):13289–13306. doi: 10.1021/acsnano.1c03035

Figure 4. In vivo PAM images of labeled ARPE-19 cells post-transplantation into rabbit retina:

Figure 4.

(A)–(D) In vivo PAM images of CGNP clusters-labeled ARPE-19 cells post-transplantation into rabbit eyes with laser-induced RPE injury. (A) Baseline fundus photograph and PAM images acquired at 578 nm and 650 nm before the transplantation of the ARPE-19 cells. White arrows indicate the locations of retinal vessels (RVs) and photocoagulation lesions. (B–D) 2D and 3D volumetric PAM images of the ARPE-19 cells acquired at different time points post-transplantation, illustrating cell viability and migration toward photocoagulation lesions. The dotted line in (B) indicates the distribution of ARPE-19 cells post-transplantation, while the dotted circles in (A–C) show the lesion areas. The pseudo green color in PAM images acquired at 650 nm shows the distribution of the transplanted ARPE-19 cells. See Supplementary Visualization 3 for 3D volumetric presentation. (E–H) In vivo PAM images of CGNP clusters-labeled ARPE-19 cells post-transplantation into rabbit eyes without laser-induced RPE injury. (E) Fundus photograph and PAM images acquired at 578 nm and 650 nm before the transplantation of ARPE-19 cells. (F–H) 2D and 3D volumetric PAM images acquired at different time points post-transplantation, illustrating cell viability and migration in a random fashion. The dotted line in (F) indicates the distribution of cells immediately post-injection, while the dotted line in (G) illustrates cell migration over time. See Supplementary Visualization S4, S5, and S6 for 3D volumetric presentation.