Figure 3.

The aminomethylated derivative (HD2) preferentially provokes apoptosis in T24 cancer cells. Cells were exposed to compounds at various concentrations for 24 hours. A. The percentage of sub-G1 cells was determined by flow cytometry, and the results are presented as the percentage of sub-G1 cells. Values (means ± SDs) are from at least three independent experiments (*P<0.05, **P<0.01). B. T24 cells were treated with compounds, DMSO (vesicle for heliomycin), or H₂O (vesicle for the derivatives HD2 and HD3) for various concentrations, and aliquots of cell lysates were resolved by SDS-PAGE and analyzed for protein expression by Western blotting. β-actin was used as an internal loading control to monitor for equal loading. Representative images are provided from at least three independent experiments.