FIG. 3.

Nucleotide sequence of the bla_IMP-2 gene cassette of In42 and flanking regions. Initiation codons of the various ORFs are indicated, and protein translations are reported below the sequence. The nucleotide sequence of the bla_IMP gene and the amino acid sequence of the IMP-1 protein (28) are also reported above that of bla_IMP-2 and below that of IMP-2, respectively, with dots representing identical residues. The putative signal peptide of IMP-2, deduced with the SIGCLEAVE program, and the signal peptide of IMP-1 (21) are underlined. The bla_IMP-2 cassette boundaries are indicated by vertical arrows. The conserved recombination core sites located at the cassette boundaries and the inverse core site are boxed. The internal 2L and 2R core sites of the 59-base element (35) are overlined with arrows. The −35 and −10 hexamers of P_ant are underlined, and the two mutations in the −35 hexamer of the P_ant promoter compared to that of In1 (14) are in boldface.