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. 2022 Mar 15;12(3):938–960.

Table 3.

The anti-cancer effects of combination treatment of retinoids and other drugs

Cancer type Retinoids (concentration) Combined drug (concentration) Study Model Findings References
Breast cancer All-trans-RA (10 μM) Epirubicin (20 μM) MCF7 cells (In vitro) ● Inhibited cell migration and invasion (stronger than epirubicin) [57]
● Up-regulated GRIM-19 and down-regulated STAT3 in the JAK/STAT signaling pathway
All-trans-RA (5 μM) Docosahexaenoic acid (30 μM) ● Induced apoptosis (stronger than all-trans-RA) [59]
All-trans-RA (0.75 μM) Interferon-β (1.87 μM) ● Inhibited cell proliferation and migration [58]
Curcumin (40 μM) ● Induced apoptosis
All-trans-RA (20 μM) ω-3 free fatty acids (80 μM) ER + MCF7 and HER2 + SKBR3 cells (In vitro) ● Inhibited cell proliferation (stronger than all-trans-RA) [60]
● Induced apoptosis
All-trans-RA (10 μM) Trastuzumab (0.0687 μM) BT474 and SKBR3 cells (In vitro) ● Inhibited cell proliferation, invasion, and migration (stronger than all-trans-RA) [50]
● Down-regulated FAK and HER2 in their respective signaling pathways
Melanoma All-trans-RA (17.53 μM) Allicin (30.81 μM) CD44+ cells (In vitro) ● Inhibited cell proliferation (stronger than all-trans-RA) [54]
● Up-regulated MMP-9 in the EGFR/MAPK signaling pathway, cyclin D1 in the Wnt/β-catenin signaling pathway, and RAR in the RAR/RXR signaling pathway
All-trans-RA (12.65 μM) CD20 antibody (1 μg/mL) CD20 + cells (In vitro) ● Inhibited cell proliferation (stronger than all-trans-RA) [64]
All-trans-RA (5 μM) Paclitaxel (0.005 and 0.01 μM) A375 cells (In vitro) ● Inhibited cell proliferation (stronger than paclitaxel) [62]
All-trans-RA Vorinostat A375, HL60, LN229, L929, SH-SY5Y and U-118 MG cells (In vitro) ● Inhibited cell proliferation (stronger than all-trans-RA) [63]
(In combination: 12.5 and 25 μM) ● Induced apoptosis
All-trans-RA Vorinostat Xenograft tumor mice (In vivo) ● Suppressed tumor development
(In combination: 10 mmol/kg (body weight))
All-trans-RA (150 mg/m2) Ipilimumab (10 mg/kg) Phase II human clinical trial ● Decreased the frequency of circulating myeloid-derived suppressor cells [65]
● Suppressed the frequency of occurrence for Grade 3 or 4 adverse effects
Colorectal cancer All-trans-RA Oxaliplatin SW-480 cells (In vitro) ● Inhibited cell proliferation [67]
(Nanoparticles: 5 μg/mL) ● Induced apoptosis
Hepatocellular carcinoma All-trans-RA (10 μM) Interferon-β (4.99 μM) HepG2 cells (In vitro) ● Inhibited cell proliferation (stronger than all-trans-RA and interferon-β) [69]
● Induced apoptosis
● Down-regulated JAK2 and STAT3 in the JAK/STAT signaling pathway
All-trans-RA Paclitaxel A549 cells (In vitro) ● Inhibited cell proliferation (stronger than all-trans-RA) [70]
(In combination: 25.7 μM)
All-trans-RA (0.35 mg/kg (body weight)) Paclitaxel (2 mg/kg (body weight)) Xenograft tumor mice (In vivo) ● Suppressed tumor development
● Prolonged survival rate
Neuroblastoma All-trans-RA (5 μM) Estradiol (0.001 μM) SH-SY5Y cells (In vitro) ● Inhibited cell proliferation (stronger than all-trans-RA) [74]
Cholesterol (12.93 μM)
All-trans-RA (0.1 μM) NaB (1000 μM) SH-SY5Y and SK-N-BE cells (In vitro) ● Inhibited cell proliferation (stronger than all-trans-RA) [53]
5-Aza (1 μM) ● Up-regulated RAR in the RAR/RXR signaling pathway
All-trans-RA (0.1 μM) TNIIIA2 (3 μg/mL) IMR-32 and Kelly cells (In vitro) ● Accelerated cell differentiation (stronger than all-trans-RA and TNIIIA2) [75]
● Down-regulated N-Myc in the MYCN signaling pathway
All-trans-RA (6.67 mg/kg (body weight)) TNIIIA2 (8.34 mg/kg (body weight)) Xenograft tumor mice (In vivo) ● Suppressed tumor development (stronger than all-trans-RA)
13-cis-RA (50 μM) Poly (I:C) (50 μg/ml) SK-N-DZ cells (In vitro) ● Inhibited cell proliferation (stronger than 13-cis-RA) [45]
● Induced apoptosis
● Up-regulated mitochondrial stress response
13-cis-RA (5 mg/kg (body weight)) Poly (I:C) (10 mg/kg (body weight)) Xenograft tumor mice (In vivo) ● Suppressed tumor development through acceleration in neural differentiation and inhibition of vessel formation
Cutaneous T-cell lymphoma Tamibarotene (25 μM) MS-275 (1 μM) SeAx cells (In vitro) ● Inhibited cell proliferation (stronger than tamibarotene) [39]
● Up-regulated RAR in the RAR/RXR signaling pathway
Tamibarotene (2 mg/kg (body weight)) MS-275 (5 mg/kg (body weight)) Xenograft tumor mice (In vivo) ● Suppressed tumor development (stronger than tamibarotene)
● Prolonged survival rate
Glioma All-trans-RA (10 μM) Temozolomide (400 μM) U251 cells (In vitro) ● Inhibited cell proliferation (stronger than temozolomide) [19]
● Induced apoptosis
● Inhibited the Keap1/Nrf2/ARE signaling pathway
9-cis-RA (600 μM) Metformin (20000 μM) C6 cells (In vitro) ● Induced apoptosis (stronger than 9-cis-RA or metformin) [78]
● Up-regulated caspase-3 in the caspase signal pathway
Lung cancer All-trans-RA (10 μM) Gefitinib (15 μM) A549 and H1650 cells (In vitro) ● Inhibited cell proliferation (stronger than gefitinib) [79]
All-trans-RA (0.01 μM) CIK cells A549 and NCI-H520 cells (In vitro) ● Inhibited cell proliferation [47]
Effector: Target ratio of CIK cells ● Down-regulated BCL-2 and surviving, and up-regulated BAX in the caspase signaling pathway
(20:1)
Gastric cancer All-trans-RA (25 μM) DAPT (5 μM) AGS and MKN45 cells (In vitro) ● Inhibited cell proliferation (stronger than all-trans-RA) [80]
● Up-regulated caspase-3 in the caspase signaling pathway
All-trans-RA Podophyllotoxin MKN45 and BGC-823 cells (In vitro) ● Inhibited cell proliferation (stronger than all-trans-RA) [49]
● Induced apoptosis
(Conjugate: 0.5 μM) ● Up-regulated RAR in the RAR/RXR signaling pathway and down-regulated ERK1/2 and AKT in the PI3K/AKT signaling pathway
Thyroid cancer All-trans-RA (70 μM) Sorafenib (18 μM) FTC-133 cells (In vitro) ● Inhibited cell proliferation [83]
● Down-regulated ERK2 in the ERK/MAPK signaling pathway
All-trans-RA Sorafenib BALB/c nude mice (In vivo) ● Induced apoptosis
(Polymer micelles: 10 mg/kg (body weight)) ● Induced re-differentiation of cells in tumor tissue
13-cis-RA (1.5 mg/kg) Iodine-131 (60 μg) Human clinal trial ● Reduced or stabilized tumor size after 2.5 years [82]
Pancreatic cancer All-trans-RA (50 μM) Gemcitabine (10 μM) AsPC-1 cells (In vitro) ● Inhibited cell proliferation (stronger than gemcitabine) [24]
All-trans-RA (30 μM) Gemcitabine 0.6 μM MiaPaCa-2 and TB33117 cells (In vitro) ● Inhibited cell proliferation and migration (stronger than gemcitabine) [84]
● Down-regulated PAK in the PAK signaling pathway
All-trans-RA (45 mg/m2) Paclitaxel (125 mg/m2) Phase I human clinical trial ● Grade 4 of Dose-Limiting Toxicity [85]
● Achieved survival duration of 11.7 months