Figure 2.

High throughput drug screening to identify candidates for overcoming venetoclax resistance. (A) Schematic illustration of the high-throughput drug screen. The drug screen was performed over a library consisting of 320 drugs in two parental and venetoclax-resistant paired cell lines by a 72 h cell viability test with one dose (5 μM) as first round screen followed by 2^nd round drug validation via 4-dose viability assay in the same cell lines to identify the top candidates in overcoming venetoclax resistance. (B) 1^st round drug screen at 5 μM by a cell viability inhibition assay in Mino, Mino-Re, Rec-1 and Rec1-Re cells. Each treatment for the cell viability assay was set up in triplicate. Relative cell viability was normalized to DMSO control. Cell viability less than 20% was considered as positive (red box). (C) 2^nd round drug validation for the top candidates (six drugs) was performed by a four-dose (three-fold dilution) cell viability assay at 72 h in the same cell lines. The heatmap shown represents the IC₅₀ values (µM). Each treatment for the cell viability assay was set up in triplicate and the experiment was repeated three times.