Figure 5.

PI3K inhibitor PIK-75 blocks in vivo homing of MCL cells to mouse spleen. (A) CMFDA-labelled patient cells (PT2) were pre-treated with vehicle (DMSO) or PIK-75 at 100 nM for 30 min before tail vein inoculation into NSG mice. Blood, spleen, and bone marrow were collected at 16 hours post inoculation for tumor cell detection by flow cytometry based on CMFDA labeling (x-axis). (B) Percentage of tumor cells was quantified based on flow cytometry analysis. Student t test was used for statistical analysis, n=5.