Figure 6.

PIK-75 overcomes primary venetoclax resistance in mouse xenograft models. (A) JeKo-1 cells were treated with venetoclax at 100 nM or PIK-75 at 10 nM for 24 hours. Cells were harvested and analyzed by western blotting. (B-D) 5×10⁶ luciferase-expressing JeKo-luc cells were injected subcutaneously into each NSG mouse and treated with vehicle, 50 mg/kg venetoclax, or 10 mg/kg PIK-75 daily. Tumor load was measured weekly by bioluminescence imaging (B) and total flux was calculated based on live imaging data (C). Tumor weight was measured at the end of the experiment (D). Student t test was used for statistical analysis, n=5.