Effect of bulk and nano forms of PFPS on the kinetics parameters of HEWL amyloid fibrillation.
| Lag time (h) | t 50 (h) | Amplitude × 10−3 | |
|---|---|---|---|
| Control | 79.44 ± 1.81 | 110.88 ± 1.34 | 8990 ± 272.88 |
| Bulk 50 μg mL−1 | 101.89 ± 3.29 | 130.07 ± 3.91 | 6770 ± 77.88 |
| Bulk 150 μg mL−1 | 102.92 ± 1.50 | 128.21 ± 3.31 | 6000 ± 92.73 |
| Bulk 225 μg mL−1 | 90.12 ± 2.10 | 113.55 ± 1.19 | 4940 ± 71.18 |
| Nano 50 μg mL−1 | 102.11 ± 1.63 | 122.61 ± 1.15 | 3430 ± 43.20 |
| Nano 150 μg mL−1 | 128.51 ± 3.00 | 148.61 ± 2.03 | 3660 ± 77.88 |
| Nano 225 μg mL−1 | —a | 165.1 ± 2.88 | 95.94 ± 1.49 |
a
The lag time could not be determined in the presence of 225 μg mL−1 nano form of PFPS since aggregation was not observed.