Assessment of the accuracy and precision of HPTLC-densitometry quantification.

Samples	Spiked (ng per band)	Detected (ng per band)	Recoverya (%)	RSDa	Average Recovery (%)
Sample 1	50	49.07 ± 1.11	98.14 ± 2.22	2.19	93.35
	100	89.79 ± 3.10	89.79 ± 3.10	3.39
	150	138.17 ± 3.65	92.11 ± 2.43	2.61
Sample 2	50	45.76 ± 2.43	91.53 ± 4.86	5.13	92.90
	100	94.57 ± 1.87	94.57 ± 1.87	1.95
	150	138.90 ± 3.88	92.60 ± 2.59	2.76
Sample 3	50	45.82 ± 1.35	91.65 ± 2.69	2.84	94.32
	100	94.55 ± 2.42	94.55 ± 2.42	2.52
	150	145.14 ± 3.79	96.76 ± 2.53	2.58
Sample 4	50	45.55 ± 1.50	91.10 ± 3.00	3.19	89.97
	100	87.75 ± 1.50	87.75 ± 1.50	1.68
	150	136.59 ± 2.52	91.06 ± 1.68	1.82

^aThe value was the average of 3 parallel measurements.