Real-time PCR analysis of TP53 and APC expressions in rectal mucosal biopsy specimens. The expression level of each gene normalized to that of GAPDH is presented as a box-and-whisker plot; the central box covers the interquartile range, with the median indicated by the line within the box. The whiskers extend to the maximum and minimum values. A, pediatric active UC cases; B, pediatric UC cases in remission; C, pediatric irritable bowel syndrome cases (control). UC, ulcerative colitis; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PCR, polymerase chain reaction.