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| Compound | LOD (μg mL−1) | LOQ (μg mL−1) | Linearity | Range (μg mL−1) | |
|---|---|---|---|---|---|
| Linear correlation coefficient (R2) | Linear regression equation | ||||
| Avanafil | 0.10 | 0.30 | 0.9993 | y = 9.2395x − 0.4212 | 0.3–3.0 |
| Imp-A | 0.0125 | 0.0375 | 0.9995 | y = 15.701x + 4.3281 | 0.3–2.25 |
| Imp-B | 0.05 | 0.15 | 0.9998 | y = 14.677x + 1.2176 | 0.3–2.25 |
| Imp-C | 0.05 | 0.15 | 0.9999 | y = 9.4336x − 0.1289 | 0.3–2.25 |
| Imp-D | 0.05 | 0.15 | 0.9994 | y = 9.7574x + 0.1656 | 0.3–2.25 |
