Table 3. Structural Information about the LNCs as Determined by SAXS, SANS, and DLS Techniques.
| parameter | unloaded LNCs | DF003-loaded LNCs |
|---|---|---|
| P(r) analysis of small-angle X-ray scattering (SAXS) data | ||
| Rg (nm) | 17.3 ± 0.09 | 15.8 ± 0.05 |
| Dmax (nm) | 49 ± 0.5 | 46 ± 0.5 |
| shape model analysis of small-angle X-ray scattering (SAXS) data | ||
| core radius (nm) | 21.7 ± 0.2 | 18.5 ± 0.2 |
| shell thickness (nm) | 2.6 ± 0.1 | 3.6 ± 0.1 |
| total radius (nm) | 24.3 ± 0.3 | 22.1 ± 0.3 |
| volume fractiona | 0.092 | 0.092 |
| polydispersity | 0.20 | 0.35 |
| χ2 | 2.4 | 3.7 |
| small-angle neutron scattering (SANS) | ||
| core radius (nm) | 20.0 ± 0.9 | 20.2 ± 0.6 |
| shell thickness (nm) | ≤1.5 | ∼2 ± 0.5 |
| total radius (nm) | 21.5 ± 0.9 | 22.2 ± 1.1 |
| shell hydration (%) | 50 | 70 |
| volume fractionb | 0.009 | 0.009 |
| polydispersity | 0.20 | 0.25 |
| SLD of shell | 3.3 | 4.5 |
| dynamic light scattering (DLS) | ||
| hydrodynamic radius (nm) | 30.0 ± 1.0 | 36.0 ± 1.0 |
| polydispersity | 0.04 ± 0.02 | 0.07 ± 0.01 |
| zeta potential (mV) | –3.7 ± 1.6 | –13.6 ± 0.8 |
a
Volume fractions estimated using a core–shell sphere model and the values are in close approximation to a theoretical volume fraction of 0.062.
b
Volume fractions estimated using a core–shell sphere model and the values are in close approximation to a theoretical volume fraction of 0.0062.