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. 2022 Mar 28;25(5):186. doi: 10.3892/mmr.2022.12702

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Copyright: © Park et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — miRNA microarray screening. Quantitative PCR of 7 miRNAs in (A) patients and (B) cells. The expression levels of miR-423 in patients with AF and the AF cell model were significantly lower than those in the PSVT and control cell groups, respectively. (C) Representative line-scan of time-lapse calcium imaging of cells loaded with the intracellular calcium indicator (Fluo-4 AM). Representative records of spontaneous Ca²⁺ transients according to the group. (D) Summary of the Ca²⁺ transient amplitude and frequency. The amplitude was significantly increased in control + miR-423 inhibitor, Ang II and Ang II + miR-423 inhibitor groups. *P<0.001 vs. control group n=5. AF, atrial fibrillation; Ang II, angiotensin II; CTL, Control; inh., inhibitor; miRNA/miR, microRNA; PSVT, paroxysmal supraventricular tachycardia; Fluo-4 AM, 4-(6-Acetoxymethoxy-2,7-difluoro-3-oxo-9-xanthenyl)-4-methyl-2,2-(ethylenedioxy)dianiline-N,N,N,N-tetraacetic acid tetrakis(acetoxymethyl) ester.