REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Monoclonal mouse anti-StrepII	Qiagen	cat#34850
Monoclonal mouse anti-His	Genescript	cat#A00186
Monoclonal mouse anti-LPS	HycultBiotech	cat#HM6011
Polyclonal rabbit anti-BamD	Pavlova et al., 2013	N/A
Polyclonal rabbit anti-EspP β-barrel (C-term peptide)	Szabady et al., 2005	N/A
Goat anti-mouse Ig 800CW IRDye	Licor	cat#926-32210
Goat anti-rabbit Ig 680LT IRDye	Licor	cat#926-680210
Bacterial and virus strains
E. coli B strain BL21(DE3)	Invitrogen	cat#C600003
E. coli K-12 strain XL1-Blue	Agilent	cat#200236
E. coli K-12 strain NEB5alpha	NEB	cat#C2987H
Chemicals, peptides, and recombinant proteins
4,4’-dipyridyl disulfide	Sigmaaldrich	cat#143057-5G
Proteinase K	Roche	cat#3115852001
Xiran SL30010P20	Orbiscope	cat#SMALP200
Deposited data
BAM-MBP-76EspP high-resolution structure	This paper	EMDB-26114, PDB: 7TTC
BAM-MBP-76EspP class 1 structure	This paper	EMDB-26105, PDB: 7TSZ
BAM-MBP-76EspP class 2 structure	This paper	EMDB-26106, PDB: 7TT0
BAM-MBP-76EspP class 3 structure	This paper	EMDB-26108, PDB: 7TT2
BAM-MBP-76EspP class 4 structure	This paper	EMDB-26107, PDB: 7TT1
BAM-MBP-76EspP class 5 structure	This paper	EMDB-26109, PDB: 7TT3
BAM-MBP-76EspP class 6 structure	This paper	EMDB-26110, PDB: 7TT4
BAM-MBP-76EspP open-sheet EspP state structure	This paper	EMDB-26111, PDB: 7TT5
BAM-MBP-76EspP intermediate-open EspP state structure	This paper	EMDB-26112, PDB: 7TT6
BAM-MBP-76EspP barrelized EspP/ continuous open BamA state structure	This paper	EMDB-26113, PDB: 7TT7
Oligonucleotides
See Table S2 in Supplemental Information	This paper	N/A
Recombinant DNA
See Table S2 in Supplemental Information	This paper	N/A
Software and algorithms
UCSF Chimera	Pettersen et al., 2004	https://www.cgl.ucsf.edu/chimera/
UCSF ChimeraX	Pettersen et al., 2021	https://www.cgl.ucsf.edu/chimerax/
RELION 3.1	Zivanov et al., 2018	https://relion.readthedocs.io/en/latest/Inst allation.html
cryoSPARC	Punjani et al., 2020	https://cryosparc.com
Coot	Emsley et al., 2010	https://www2.mrclmb.cam.ac.uk/personal/pemsley/coot/
Isolde	Croll, 2018	https://isolde.cimr.cam.ac.uk
Rosetta	Wang et al., 2016	https://www.rosettacommons.org/software
Phenix	Adams et al., 2010	https://phenixonline.org
Other
OPM server	Lomize et al., 2012	https://opm.phar.umich.edu/ppm_server