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. Author manuscript; available in PMC: 2022 Apr 6.
Published in final edited form as: Science. 2021 Oct 29;374(6567):eabb3420. doi: 10.1126/science.abb3420

Fig. 1. p21-activated Rb interacts with STAT and SMAD TFs at select gene promoters to establish a bioactive secretome.

Fig. 1.

(A) Venn diagrams of RNA-seq data depicting downregulated SASP factors with depletion of p21 or Rb in the indicated SNCs. (B) Heatmaps of commonly downregulated SASP factors indicated in (A). FC, fold change. (C) Identification of TFs that transcriptionally activate genes encoding PASP factors using overrepresentation analyses on RNA-seq data from IR-, REP, OI-senescent MEFs, IR-senescent IMR-90 cells, and their non-senescent counterparts. Bolded TFs are significantly activated in SNCs and inhibited upon shp21 and shRb. FDR, false discovery rate. (D) Identification of SASP genes that bind Rb, and TF motif analysis of Rb peaks underlying SFs in OI-senescent IMR-90 cells. (E) Representative Rb occupancy plots at PASP genes. Experiments in the above panels were performed once.