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. Author manuscript; available in PMC: 2022 Apr 6.
Published in final edited form as: Science. 2021 Oct 29;374(6567):eabb3420. doi: 10.1126/science.abb3420

Fig. 3. p21-induced immunosurveillance requires PASP factor CXCL14.

Fig. 3.

(A) Transwell migration assay with CM in the presence of CXCL14-neutralizing or IgG antibodies. (B) as in (A) but with CM from shRNA-transduced MEFs. (C) Schematic of the transgenes used for Cre-inducible expression of Myc-Flag-tagged p21 (L-p21 for LoxP/Stop/LoxP-p21) and tdTomato (Ai14) (Top) and the experimental setup for p21-OE induction in hepatocytes via Cre-encoding adenovirus injection (Bottom). (D) RT-qPCR on flow-sorted Tom+ hepatocytes. (E) Representative picture and quantification of Tom+ hepatocytes joined by ≥3 F4/80+ cells. (F) As in (E) but assessing livers from mice treated with CXCL14-neutralizing or IgG control antibodies. Scale bars: 10 μm (E). Data represent means ± SEM. Experiments in the above panels were performed once. ns, not significant. *P<0.05; **P<0.01; ***P<0.001. One-way ANOVA with Sidak’s correction (A, B, E, and F) and unpaired two-tailed t tests (D).