|
| Antibodies |
|
| CaMKII pan |
Genetex |
GTX127939; RRID: AB_2492051 |
| CaMKIIα |
Made in house |
CBα2; RRID: AB_2533032 |
| CaMKIIβ |
Made in house |
CBβ1; RRID: AB_2533045 |
| pT286 |
Phospho-Solutions |
p1005–286; RRID: AB_2492051 |
| Rabbit |
GE Healthcare |
NA934V; RRID: AB_772206 |
| Mouse |
GE Healthcare |
NA931V; RRID: AB_772210 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Glutamate |
Sigma |
6106-04-3 |
| Papain |
Worthington |
LS 03126 |
| Lipofectamine 2000 |
Invitrogen |
11668027 |
| B-27 supplement |
GIBCO |
17504044 |
| complete protease inhibitor cocktail |
Roche |
1187380001 |
| Microcystin-LR |
Calbiochem |
475815 |
| Uranyl formate |
SPI-Chem |
16984-59-1 |
| Sodium hydroxide |
Fisher Scientific |
1310-73-2,497-19-8 |
| Calmodulin |
Made in house |
CaM |
| Ca2+/CaM-dependent kinase II α |
Made in house |
CaMKII α |
| Ca2+/CaM-dependent kinase II β |
Made in house |
CaMKIIβ |
|
| Critical commercial assays |
|
| Pierce BCA protein assay |
Thermo Fisher |
23225 |
| SuperSignal West Femto |
Thermo Fisher |
34095 |
|
| Deposited data |
|
| Raw and analyzed data |
This paper |
Mendeley Data, V1, https://doi.org/10.17632/35gf4sjxmb.1
|
|
| Experimental models: Organisms/strains |
|
| Rat: Sprague-Dawley |
Charles River Laboratory |
N/A |
| Mouse: wild type: C57BL/6 |
Charles River Laboratory |
N/A |
| Mouse: CaMKIIα KO: C57BL/6 |
(Coultrap et al., 2014) |
N/A |
|
| Recombinant DNA |
|
| PSD-95-FingR-GFP |
(Gross et al., 2013) |
RRID: Addgene_46295 |
| Gephyrin-FingR-GFP |
(Gross et al., 2013) |
RRID: Addgene_46296 |
| YFP-CaMKIIα |
(Bayer et al., 2006; Shen and Meyer, 1999) |
N/A |
| YFP-CaMKIIβ |
Made in house |
N/A |
|
| Software and algorithms |
|
| Slidebook 6.0 |
Intelligent Imaging Innovations (3i) |
RRID:SCR_014300 |
| Prism 7.0 |
Graphpad |
RRID: SCR_002798 |
| AlphaEase FC 4.0 |
Alpha Innotech |
N/A |
| ImageJ |
NIH |
RRID:SCR_003070 |
| EMAN2.1 |
NIH |
RRID:SCR_018867 |
| RELION3.0b |
MRC (United Kingdom) |
RRID:SCR_016274 |
| UCSF Chimera |
NIH |
RRID:SCR_004097 |
| Python 3 |
Python Software Foundation |
RRID:SCR_008394 |
| Microsoft Excel |
Microsoft |
RRID:SCR_016137 |
|
| Other |
|
| 400 mesh, 3.0 mm O.D. copper grid |
PELCO |
1GC400 |
