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. Author manuscript; available in PMC: 2023 Feb 22.
Published in final edited form as: Anal Chem. 2022 Feb 10;94(7):3328–3334. doi: 10.1021/acs.analchem.1c05398

Figure 2.

Figure 2.

TMT reporter (130N) signal generated from the ten most abundant b-type ions resulting from 30% HCD of [OVA + 130N + 3H]3+ at various (a) HCD offset voltages and (b) IR laser powers. A 20 V parking waveform was used to preserve the TMT reporter (q = 0.8) during IRMPD. Insets illustrate that (a) the optimal HCD offset for reporter generation varies with precursor b ion m/z and (b) the optimal IR power for reporter generation is constant with respect to precursor b ion m/z. (c) Percent increase of TMT reporter generated at optimal IR power (see (b) inset) over reporter generated at optimal HCD voltage (see (a) inset) for the ten b-type ions.