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. Author manuscript; available in PMC: 2023 Feb 22.
Published in final edited form as: Anal Chem. 2022 Feb 10;94(7):3328–3334. doi: 10.1021/acs.analchem.1c05398

Figure 4.

Figure 4.

Back-to-back analysis of using IRMPD vs. HCD for generation of reporter ions from the standard TKO yeast standard. Both experiments used HCD (30 nce) to generate fragments from individually isolated peptides and either 50 nce HCD or 40% IR for 5ms to generate TMT reporter from 10 synchronously selected peptide fragments. A 20 V parking waveform was applied during IRMPD to minimize loss of reporter due to photodissociation. (a) Ratio of reporter signal generated via IRMPD over HCD vs. yeast peptide m/z. (b) Histogram of reporter signal increase due to IRMPD over HCD for all peptides.