Fig. 2.

Secretome from 4T1 metastatic mammary cancer cells promotes microglial proliferation, directional migration, and activation. a Representative brightfield images of primary adult mouse microglia. b Growth curves of BV-2 microglial cells upon exposure to SFM, 4T07 CM and 4T1 CM analyzed using MTT assay. c Microglial cells were plated in the upper compartment of a boyden chamber chemotaxis assay, with either SFM, 4T07 CM or 4T1 CM the as chemoattractant for 24 h. Representative brightfield images of migrated BV-2 microglial cells captured at 100× magnification and quantification of migrated cells after 24 h. d BV-2 microglia were treated with either SFM, 4T07 CM or 4T1 CM and gene expression of inflammatory markers was determined by qRT-PCR. Data represent mean ± SEM; n = 3 independent experiments. Brightfield images captured at 100× magnification, representative of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001