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. 2022 Apr 5;24:25. doi: 10.1186/s13058-022-01514-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — ANXA1 in 4T1 metastatic mammary cancer cells secretome promotes BV-2 microglial directional migration. a–d BV-2 microglial cells were treated with indicated concentrations of a SFM, 4T1 CM or 4T1 ΔANXA1 CM, b recombinant ANXA1 protein, c 4T1 CM supplemented with Boc-MLF FPR1 antagonist or d WRW4 FPR2 antagonist for 24 h in transwell migration assay. Data represent mean ± SEM; n = 3 independent experiments. Images are representative of three independent experiments. ****P < 0.0001