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. 2022 Apr 5;24:25. doi: 10.1186/s13058-022-01514-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Extracellular and intracellular ANXA1 elicited different effects on migratory profiles in BV-2 microglial cells. a Primary adult microglia treated with either SFM, 4T07 CM, or 4T1 CM for 24 h and stained with ANXA1 (green), microglial marker Iba1 (red), and DNA-binding dye DAPI (blue). Graph (rightmost) showing the immunoreactivity against ANXA1 on primary adult microglial cells. b and c BV-2 or BV-2 ΔANXA1 microglia were treated with SFM, 4T1 CM or 4T1 ΔANXA1 CM for 24 h in the transwell migration assay. Representative brightfield images and quantification of migrated BV-2 or BV-2 ΔANXA1 microglia. c BV-2 or BV-2 ΔANXA1 microglia were treated with SFM, 4T1 CM or 4T1 ΔANXA1 CM for 24 h and gene expression of inflammatory markers were analyzed using qRT-PCR. Data represent mean ± SEM; n = 3 independent experiments. *P < 0.05, **P < 0.01