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. 2022 Apr 5;24:25. doi: 10.1186/s13058-022-01514-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — ANXA1 regulates STAT3 signaling through FPRs in microglia. a, c and d BV-2 microglia were treated with SFM, 4T1 CM, 4T1 ΔANXA1 CM or 4T1CM with the indicted inhibitors for 24 h. b BV-2 microglia were treated with SFM or 4T1 CM for 24 h. c BV-2 or BV-2 ΔANXA1 microglial cells were treated with SFM, 4T1 CM or 4T1 ΔANXA1 CM for 24 h. Indicated proteins were analyzed using western-blot analysis. f BV-2 microglia were treated with SFM or 4T1 CM for 24 h. The interaction between ANXA1 and phosphorylated STAT3 was assessed using immunoprecipitation (IP). Images shown are representative of at least 3 independent biological replicates. Loading control: β-actin