Optimization of a sequential one-pot process with PDMS thimble.

Entry	Chamber	rac-1a [mmol]	Mn oxidant [g]	CH2Cl2 [mL]	Enzyme [U mmol−1]	NADP+ [μmol]	Buffer [mL]	IPA [mL]	Yielda [%] (eeb)
									2a	(R)-1a
1	Interior	1	2	4	—	—	—	—	19	4 (93% ee)
	Exterior	—	—	—	120	20	15	5	42	31 (>99% ee)
2	Interior	1	2	4	—	—	—	—	n.d.c	n.d.c
	Exterior	—	—	—	120	20	15	15	35	57 (>99% ee)
3	Interior	1	2	4	—	—	—	—	n.d.c	n.d.c
	Exterior	—	—	—	500	20	15	15	13	86 (>99% ee)
4	Interior	1	0.5	4	—	—	—	—	n.d.c	n.d.c
	Exterior	—	—	—	500	20	15	15	3	95 (>64% ee)
5	Interior	1	1	4	—	—	—	—	n.d.c	n.d.c
	Exterior	—	—	—	500	20	15	15	4	96 (>99% ee)
6	Interior	1	1.5	4	—	—	—	—	n.d.c	n.d.c
	Exterior	—	—	—	500	20	15	15	6	91 (>99% ee)
7	Interior	1	3	4	—	—	—	—	n.d.c	n.d.c
	Exterior	—	—	—	500	20	15	15	14	84 (>99% ee)

^aYield was calculated from ¹H-NMR.

^bEnantiomeric excess of (R)-1-phenylethanol was determined by chiral HPLC.

^cn.d.: not detected.