Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Acquire a confocal image of the nascent matrix, cell membrane and nucleus, and split the channels into single images using ImageJ (scale bar 10 μm). (i). To obtain an image of the nascent matrix only, adjust the threshold for each channel with ‘Otsu thresholding’ and subtract the ‘cell’ image from the ‘nascent matrix’ image (ii). Use the ImageJ plugin ‘BoneJ’ to measure the average nascent matrix thickness (iii).
