. Author manuscript; available in PMC: 2023 Mar 1.

Published in final edited form as: Nat Protoc. 2022 Feb 9;17(3):618–648. doi: 10.1038/s41596-021-00652-9

Table 1.

Recipe for preparing AHA media (5 mL per condition, values listed in μL)

AHA concentration	25%	50%	75%	100%

DMEM	4335	4340	4338	4335
AHA	2.5	5.0	7.5	10
L-Methionine	1.125	0.75	0.375	0
L-Cystine	4.83	4.83	4.83	4.83
Glutamax	50	50	50	50
Ascorbic acid	5	5	5	5
FBS	500	500	500	500
Penicillin/Streptomycin	50	50	50	50
Sodium Pyruvate	45	45	45	45