Figure 2.

Larger hypoperfusion area and higher mortality in eMCAO. Mice were subjected to eMCAO induced by a 10 mm blood clot or transient MCAO induced by intraluminal suture (sMCAO). (a) Representative 2D laser speckle images of sMCAO and eMCAO pre-ischemia and at indicated time points after surgery. (b) Quantification of core (CBF ≤ 30% of baseline) and peri-core (30% < CBF ≤ 50% of baseline) area in sMCAO and eMCAO at indicated time points after ischemia. n = 13 per group. **p ≤ 0.01, ***p ≤ 0.001 vs. sMCAO at the same time points using two-tailed Student’s t test or Mann–Whitney test. (c) Quantification of the total infarct volume, and infarct volume in each region, including cortex (CTX), striatum (STR), hippocampus (HIP), thalamus (THA), and deutocerebrum (DEU) 24 h after MCAO by TTC staining. n = 13 per group. *p ≤ 0.05, ***p ≤ 0.001 vs. sMCAO using the two-tailed Student’s t test. (d) Sensorimotor dysfunctions were assessed by corner test and rotarod test within seven days after surgery, n = 7–8 per group. ns: no significant difference, **p ≤ 0.01, ***p ≤ 0.001 vs. sham using two-way RM ANOVA followed by Bonferroni post hoc test. (e) Atrophy/infarct volume was measured by NeuN staining seven days after MCAO, n = 7–8 per group. ns: no significant difference analyzed by a two-tailed Student’s t test. (f) NeuN staining of sMCAO (60 min) and eMCAO seven days after surgery. Scale bar = 2 mm. The white dashed line circles the infarct area, while the red dashed line outlines the relative area of the contralateral hemisphere to illustrate atrophy area in the ipsilateral hemisphere. (g) Kaplan–Meyer curves illustrating the survival of mice after sMCAO and eMCAO. n = 30–34 per group. **p ≤ 0.01 using Long-Rank test for Kaplan–Meyer curves. All data are presented as mean ± SD.