Fig. 4. Intact mass analysis of the trastuzumab-DOTA conjugate and its subunits by LC-FTMS. The deconvolved mass spectra of the intact deglycosylated sample (A) and deglycosylated TCEP reduced sample, yielding light (B) and heavy chains (C); GingisKHAN™ derived Fc (D) and Fab (E) subunits. The D0–D4 peaks correspond to the antibody or antibody fragment with different degrees of conjugation, where Dn is the number of attached DOTA molecules.
