Skip to main content
. Author manuscript; available in PMC: 2022 Apr 6.
Published in final edited form as: Nat Biotechnol. 2021 Jun 28;39(11):1414–1425. doi: 10.1038/s41587-021-00938-z

Figure 3. Effect of varying the cytidine deaminase and Cas9 components of CGBEs on C•G-to-G•C editing outcomes in HEK293T cells.

Figure 3.

(a) C•G-to-G•C editing outcomes for catalytically impaired, narrow-window cytidine deaminases show higher editing purity at HEK2 and RNF2. (b) C•G-to-G•C editing outcomes for high-fidelity Cas9 variants show altered editing windows and improved CGBE performance at some positions. “Cas9” represents the Cas9 D10A nickase variant of each Cas effector. Values and error bars reflect the mean and standard deviation of three biological replicates, shown as individual data points. HEK2=HEK site 2; HEK3=HEK site 3; HEK4=HEK site 4. C4, C6, and similar annotations indicate the in-window target nucleotides where the SpCas9 PAM is at positions 21–23.