Figure 2.

The ‘backbone’ of the FUSION screen, formed with the four precipitant mixes (P1–P4) and three titrated buffer systems (pH1–pH3) from the original MORPHEUS screen. For example, the combination {P1,pH1} in well A1 corresponds to ‘precipitant mix 1’ and ‘buffer system 1’, titrated to pH 6.5 (Table 3 ▸). The combination ‘{P1, pH1}’ is shown in bold to indicate the shift in positions across the 12 columns, in contrast to the original MORPHEUS 3D grid screen, where a single combination of precipitant and buffer populates each column (Gorrec, 2009 ▸). As a result, there are four of each mix of precipitants and three of each buffer system across each row, while there are two of each mix of precipitants and two to three of each buffer system in each column.