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. 2022 Mar 11;55(Pt 2):310–319. doi: 10.1107/S1600576722001765

Table 1. Formulation of FUSION set 1 of additive mixes.

The stock solutions were supplied by Molecular Dimensions Ltd (Sheffield, UK).

Mix name Label Chemicals Catalogue No. Origin
Divalent metal cations 1 1 0.3 M magnesium chloride, 0.3 M calcium chloride MD2-100-70 MORPHEUS I
Divalent metal cations 2 2 5 mM manganese chloride, 5 mM cobalt chloride, 5 mM nickel chloride, 5 mM zinc acetate MD2-100-232 MORPHEUS II
NPS 3 0.3 M sodium nitrate, 0.3 M disodium hydrogen phosphate, 0.3 M ammonium sulfate MD2-100-72 MORPHEUS I
Carb­oxy­lic acids 4 0.2 M sodium formate, 0.2 M ammonium acetate, 0.2 M tri­sodium citrate, 0.2 M sodium potassium L-tartrate, 0.2 M sodium oxamate MD2-100-76 MORPHEUS I
Amino acids 5 0.2 M sodium L-glutamate, 0.2 M DL-alanine, 0.2 M glycine, 0.2 M DL-lysine HCl, 0.2 M DL-serine MD2-100-77 MORPHEUS I
LiNaK 6 0.3 M lithium sulfate, 0.3 M sodium sulfate, 0.3 M potassium sulfate MD2-100-231 MORPHEUS II
Halides 7 0.3 M sodium fluoride, 0.3 M sodium bromide, 0.3 M sodium iodide MD2-100-71 MORPHEUS I
Alkalis 8 10 mM rubidium chloride, 10 mM strontium acetate, 10 mM caesium acetate, 10 mM barium acetate MD2-100-233 MORPHEUS II
Oxometallates 9 5 mM sodium chromate, 5 mM sodium molybdate, 5 mM sodium tungstate, 5 mM sodium orthovanadate MD2-100-234 MORPHEUS II
Vitamins 10 3% w/v sodium L-ascorbate, 3% w/v choline chloride, 3% v/v D-panthenol, 3% w/v pyridoxine hydro­chloride, 3% w/v thi­amine hydro­chloride MD2-100-314 MORPHEUS III
Polyamines 11 0.1 M spermine·4HCl, 0.1 M spermidine·3HCl, 0.1 M 1,4-di­amino­butane·2HCl, 0.1 M DL-ornithine·HCl MD2-100-238 MORPHEUS II
Anaesthetic alkaloids 12 2% w/v lidocaine hydro­chloride monohydrate, 2% w/v procaine hydro­chloride, 2% w/v proparacaine hydro­chloride, 2% w/v tetracaine hydro­chloride MD2-100-320 MORPHEUS III