Table 1. Formulation of FUSION set 1 of additive mixes.
The stock solutions were supplied by Molecular Dimensions Ltd (Sheffield, UK).
Mix name | Label | Chemicals | Catalogue No. | Origin |
---|---|---|---|---|
Divalent metal cations 1 | 1 | 0.3 M magnesium chloride, 0.3 M calcium chloride | MD2-100-70 | MORPHEUS I |
Divalent metal cations 2 | 2 | 5 mM manganese chloride, 5 mM cobalt chloride, 5 mM nickel chloride, 5 mM zinc acetate | MD2-100-232 | MORPHEUS II |
NPS | 3 | 0.3 M sodium nitrate, 0.3 M disodium hydrogen phosphate, 0.3 M ammonium sulfate | MD2-100-72 | MORPHEUS I |
Carboxylic acids | 4 | 0.2 M sodium formate, 0.2 M ammonium acetate, 0.2 M trisodium citrate, 0.2 M sodium potassium L-tartrate, 0.2 M sodium oxamate | MD2-100-76 | MORPHEUS I |
Amino acids | 5 | 0.2 M sodium L-glutamate, 0.2 M DL-alanine, 0.2 M glycine, 0.2 M DL-lysine HCl, 0.2 M DL-serine | MD2-100-77 | MORPHEUS I |
LiNaK | 6 | 0.3 M lithium sulfate, 0.3 M sodium sulfate, 0.3 M potassium sulfate | MD2-100-231 | MORPHEUS II |
Halides | 7 | 0.3 M sodium fluoride, 0.3 M sodium bromide, 0.3 M sodium iodide | MD2-100-71 | MORPHEUS I |
Alkalis | 8 | 10 mM rubidium chloride, 10 mM strontium acetate, 10 mM caesium acetate, 10 mM barium acetate | MD2-100-233 | MORPHEUS II |
Oxometallates | 9 | 5 mM sodium chromate, 5 mM sodium molybdate, 5 mM sodium tungstate, 5 mM sodium orthovanadate | MD2-100-234 | MORPHEUS II |
Vitamins | 10 | 3% w/v sodium L-ascorbate, 3% w/v choline chloride, 3% v/v D-panthenol, 3% w/v pyridoxine hydrochloride, 3% w/v thiamine hydrochloride | MD2-100-314 | MORPHEUS III |
Polyamines | 11 | 0.1 M spermine·4HCl, 0.1 M spermidine·3HCl, 0.1 M 1,4-diaminobutane·2HCl, 0.1 M DL-ornithine·HCl | MD2-100-238 | MORPHEUS II |
Anaesthetic alkaloids | 12 | 2% w/v lidocaine hydrochloride monohydrate, 2% w/v procaine hydrochloride, 2% w/v proparacaine hydrochloride, 2% w/v tetracaine hydrochloride | MD2-100-320 | MORPHEUS III |