Extended Figure 4. Terminal patterning controls MyoII activation in the posterior endoderm:

(a) Schematics of the terminal patterning-dependent pathway controlling MyoII activation in the posterior endoderm. (b) MyoII activation in the posterior endoderm in embryos mutant for torso (mat tor−/−), depleted of maternal and zygotic fog (fog RNAi) and mutant for the Gα12/13 concertina (mat cta−/−). Yellow contours mark cells in the propagation region. N=5 embryos for WT, 4 for mat tor−/−, 5 for fog RNAi and 5 for mat cta-/−. (c) Sagittal sections of embryos immunostained for Fog and Tll (together with the membrane marker Neurotactin in the merge) at different stages of posterior endoderm morphogenesis. The white and green lines indicate the boundaries of the expression domains of fog and tll respectively. N=8 embryos for primordium contraction stages and 5 embryos for MyoII propagation stages from 1 independent experiment in this configuration.