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Rebiopy of lymphnode and NGS for resistant mechanism. A. In the lymph node resection specimen, the tumor cells showed similar histology to that of lung biopsy. B–D. The tumor cells still showed positive staining of TTF-1 on nucleus, CgA and Syn in the cytoplasma, respectively (immunohistochemistry stain, original magnification × 300). E. Targeted next-generation sequencing revealed ALK F1174L on exon 23, and two rare forms of ALK rearrangements.
