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. Author manuscript; available in PMC: 2022 Apr 6.
Published in final edited form as: Mov Disord. 2018 Nov 28;34(4):496–505. doi: 10.1002/mds.27558

FIG. 2.

FIG. 2.

Cell-based Rab10 phospho-assay comparing the Asp1887Gly (D1887G) variant with WT LRRK2, the most common PD variant Gly2019Ser (G2019S) and a kinase-dead control Lys1906Met (K1906 M). Strep/FLAG-LRRK2 variants have been coexpressed with Flag/HA-Rab10 (N = 3 biological +3 technical replicates). Statistical significance has been determined by ANOVA and Tukey’s post-hoc test. Left panel: relative Rab10 phosphorylation levels observed for different LRRK2 variants. Right panel: SDS-PAGE and western blot analysis after FLAG-IP. Rab10 phosphorylation has been detected by a phospho-pattem–specific antibody. Equal loading has been determined by colloidal Coomassie stain. For the western blot analysis and for the colloidal Coomassie stain, approximately 8 and 37 pmole of FLAG/HA-Rab10 were loaded, respectively.