Figure 2.

GluK2-containing KARs mediate KA-induced robust action potential firing of MCs. A, Top, Effect of KA bath application (0.3 and 3 μm) on the membrane potential of MCs in current-clamp configuration. The application of 3 μm KA induced firing with faster onset and higher frequency than 0.3 μm KA. Firing in 3 μm KA eventually disappeared, likely because of excessive depolarization and action potential refractoriness. Bottom, Average firing rate histogram (expressed in spikes per second) for the experiments in the top panel. Black and gray traces represent the mean and SEM of the MC firing rate. B, Representative traces showing no effect of KA application on MCs firing in GluK2 KO mice.