Table 1. Oligonucleotides and PCR cyclic conditions for PCR amplification of genes used in this study.
| Target gene | Primer | Nucleotide sequence (5’-3’) | Target gene (bp) | PCR cyclic conditions | Reference |
|---|---|---|---|---|---|
| Dlts | dlts-F | AAGTACATGCTGATCAAGT | 952 |
5 min at 94°C, 35 cycles at 94°C for 45 sec, 66°C for 45 sec, 72°C for 1.5 min, and 72°C for 5 min |
[27] |
| dlts-R | TCTTGATCAACTTGTTGTAC | ||||
| 16S rRNA | F27 | AGAGTTTGATCMTGGCTCAG | 1485 |
5 min at 95°C, 35 cycles at 94°C for 30 sec, 56°C for 60 sec, 72°C for 60 sec, and 72°C for 10 min |
[28] |
| R1492 | TACGGYTACCTTGTTACGACTT | ||||
| Pbp1A/ponA | Pbp1A/ponA-F | AGGGGTAGTAGCATTACCAT | 939 |
5 min at 95°C, 38 cycles at 95°C for 30 sec, 47°C for 30 sec, 72°C for 30 sec, and 72°C for 10 min |
[34] |
| Pbp1A/ponA-R | CAACTATATGACTGGGATCG | ||||
| Cfb | cfb-F | GGATTCAACTGAACTCCAAC | 600 | ||
| cfb-R | GACAACTCCACAAGTGGTAA | ||||
| FbsA | fbsA-F | AACCGCAGCGACTTGTTA | 278 | ||
| fbsA-R | AAACAAGAGCCAAGTAGGTC |