Fig 2. DHX38 downregulation changes primarily gene expression.

Summary of three independent RNA-seq analysis of HEK293 cells treated either with anti-DHX38 siRNA or negative control siRNA. Analysis of (A) splicing efficiency, (B) alternative splicing and (C) gene expression. Expression of selected genes that were downregulated (D) or upregulated (E) after DHX38 downregulation was analyzed by RT-qPCR. RNA was isolated from control cells or cells treated with cycloheximide (CHX) to reveal potential targets of non-sense mediated decay. Average of three independent experiment with SEM is shown. Expression value determined by RNA-seq analysis is shown in the same graph for comparison (RNA seq).