Figure 1: Schematic workflow.

Proteins extracted with detergent aid will require processing with an extra step of detergent removal before digestion. Protein samples are digested, and peptides are desalted on the OT-2 robotic system. The peptide digests are injected into a Q-Exactive HF mass spectrometer coupled with a nano-LC. MS spectra are searched against a protein database for protein identification.