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. 2022 Mar 22;7(6):e157694. doi: 10.1172/jci.insight.157694

Figure 2. Notch-induced glucose intolerance is only partially reversible.

Figure 2

(A) Experimental schematic used for Dox withdrawal (Dox-off). (B) Notch target (left) and β cell maturity gene (right) expression in islets isolated from 8 weeks’ Dox-off β-tetO-NICD and Cre– mice (n = 4 mice/group). (C) Representative images of pancreatic sections from 8 weeks’ Dox-off β-tetO-NICD and Cre– mice, with quantitation of β cell nuclear Mafa fluorescence intensity (n = 5 mice/group). Scale bars: 20 μm. (D) GTT in male and (E) female Dox-off β-tetO-NICD and Cre– controls (n = 7–9 mice/group). AUC, area under the curve (mg × dL/min). (F) Serum insulin after oral glucose challenge in Dox-off β-tetO-NICD and Cre– controls (n = 5 mice/group). (G) Glucose-stimulated insulin secretion (GSIS) in islets isolated from Dox-off β-tetO-NICD and Cre– mice, adjusted for islet insulin content (n = 5 mice/group). All data are shown with group means; *, P < 0.05, **, P < 0.01, ***, P < 0.001 by 2-tailed t test.