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. 2022 Mar 22;7(6):e153136. doi: 10.1172/jci.insight.153136

Figure 6. Increased number of CD8+ T cells adjacent to CADM1+CD68+ cells during T1D.

Figure 6

(A) Immunostaining of paraffin-embedded pancreata from individuals in the Non, aAb+, and T1D groups for CADM1 (red), CD68 (green), and CD8 (cyan). Scale bar: 50 μm. (B) Quantification of the number of CADM1+ cells adjacent to CD8+ cells within the islet boundary (n = 5 per group). (C) Pearson correlation indicates increased colocalization between Cadm1 and CD68 expression in aAb+ (n = 5) and T1D (n = 5) pancreata compared with pancreata from the Non group (n = 5). (D) Three-dimensional rendering of immunostaining of paraffin-embedded pancreata from individuals in the Non, aAb+, and T1D groups for CD8 (green) and CD68 (magenta) (left column, scale bar: 5 μm). Parent images of original immunostaining (3 right-most columns, scale bar: 20 μm). (E) Quantification of colocalized volumes of CD68 and CD8 after immunostaining of paraffin-embedded pancreata from the Non, aAb+, and T1D groups for CD68 (green) and CD8 (magenta). One-way ANOVA was performed using GraphPad Prism, version 7, software for comparisons of 3 groups. Post hoc statistical analyses were performed using the Tukey multiple comparisons test. Results are presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.