TABLE 3.

RFLP analyses of resistance gene cassettes found in class 1 integrons in V. cholerae

Gene cassette	Size (bp) of amplicon obtained with primers in-F and in-B	Restriction enzyme used	Restriction fragment sizes (bp)	GenBank no. of identical sequence	Reference
aadA2	1,009	HindIII	216, 275, 517	D43625	31
dfrA1	1,237a	ClaI	436, 801	X17477	48
dfrA15	739	HindIII	315, 424	Z83311b	2
aadB	744	SphI	175, 569	L06418	10
blaP1	1,197	MfeI	258, 939	AF071555	9

^aThe amplicon contained both the dfrA1 gene cassette and an open reading frame. See also Fig. 1. 

^bThe sequence with this GenBank no. included some of the aadA gene sequence located downstream of the dfrA15 gene cassette.