FIGURE 1.

(A) Schematic of applying cancer diagnostic probe (CDP) in real‐time detection of suspicious margins during breast cancer surgery. The assay was conducted on a suspicious margin inside the patient's body (lateral margin of patient ID: 2), which is the significance of CDP. It also positively scored the margin, and the removed specimen showed a negative result for malignancy in frozen analyses. Meanwhile, the permanent H&E showed the papillary lesion with the atypia region, which must be removed by the surgeon. Inferior IM of the other patient (ID 62) was negatively scored by CDP and confirmed by both frozen and permanent H&E as usual hyperplasia and (B) CDP as a surgeon‐assisted tool in the surgery room for finding involved IMs to pre‐invasive/invasive cells. (C) Four neighboring regions of a positive internal margin (had been scored by CDP) were checked by CDP, not only to prevent additional cutting of free lesions but also to remove remained involved regions (Movie S1)