TABLE 2.
Genotypic and phenotypic characteristics of clinical isolates resistant to IDV and NFVa
| Clinical isolate | IDV and RTV IC50 (μM) at baseline (before PI treatment) | IDV, RTV, and NFV IC50 (μM) after PI treatment | Protease genotype after PI treatment |
|---|---|---|---|
| 002 | 0.001 ± 0.0002 (0.001 ± 0.0003)b | >0.1 | K20R, L24L/I, M36I, M46M/L, L63P, V82A |
| 003 | 0.01 ± 0.0005 (0.001 ± 0.0001) | >0.1 | L10L/I, K20R, M36I, I54V, L63P, A71I/V, V82A, I84I/V, L90M |
| 006 | 0.001 ± 0.0001 (0.001 ± 0.0004) | >0.1 | Fragment not amplified |
| 001 | 0.001 ± 0.0004 (0.001 ± 0.0006) | >0.1 | L10I, L63S, V77I |
| 010 | 0.001 ± 0.0002 (0.001 ± 0.0005) | >0.1 | L10I, G48V, I54V, L63P/S, V77I, V82A, L90M |
| 004 | 0.001 ± 0.0004 (0.001 ± 0.0006) | >0.1 | L10I, L24I, M36I, M46I, I54V, L63C, A71V/A, V82A, I84V |
| 007 | >0.1 (0.001 ± 0.0002) | Not applicable | L10L/I, N37D, L63P |
| 008 | Not applicable | >0.1 | L10I, M36I, N37D, G48V, I54V, L63P, A71V, V82A, L90M |
| 005 | 0.01 ± 0.0009 (0.001 ± 0.0002) | >0.1 | L10I, K20R, M36I/M, I54V, L63Q, I64I/V, A71V, V82A, L90M |
| 009 | 0.01 ± 0.0007 (0.001 ± 0.0005) | >0.1 | L10I, K20R, M36I, F53F/L, I54V, L63L/P, I64V, V82A |
a
Results are expressed as means ± SEMs; three independent experiments were performed to derive the mean.
b
Values in parentheses are IC50s of NFV (in micromolar).