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. 2022 Mar 29;11:e75545. doi: 10.7554/eLife.75545

Figure 8. Glypican-1 (GPC1) is dispensable for fibroblast growth factor 2 (FGF2) signaling.

Figure 8.

Various forms of HeLa cells including wild-type and GPC1 knockout cells as well as cells overexpressing GPC1 in either a wild-type or a GPC1 knockout background were treated with recombinant FGF2 at the concentrations indicated. Where indicated, cells were treated with a mixture of heparinase I, II, and III used as a positive control. As a read-out for FGF signaling, the ratio between phosphorylated and unphosphorylated ERK1/2 was determined. For experimental details, see Materials and methods.

(A) Quantitative analysis of the pERK1/2 to ERK1/2 ratio (n=4; standard deviations are shown). The statistical analysis was based on a one-way ANOVA test combined with Tukey’s post hoc test (*, p ≤ 0.05; **, p ≤ 0.01, and ***, p ≤ 0.001). (B) A representative Western analysis was used for the quantification and statistical analysis shown in panel A. The GAPDH signal was used as a loading control.

Figure 8—source data 1. Raw data of the cell-based signaling experiments quantifying fibroblast growth factor 2 (FGF2)-induced signaling cascades under the conditions indicated.