Video 3. Endocytosis of recombinant fibroblast growth factor 2 (FGF2)-GFP (5 µg/ml, in green) and Transferrin-Alexa Fluor546 (25 µg/ml, in red) was visualized in HeLa S3 knockout cells overexpressing Glypican-1 (GPC1).

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One frame corresponds to 1 min. The data shown are representative for three independent experiments. Scale bar = 20 µm.