Table 1. qPCR reaction composition.

	1× Reaction Volume (µL)
Primer 1: qBALF5-F (10 µM), [final] = 0.3 µM	0.3
Primer 2: qBALF5-R (10 µM), [final] = 0.3 µM	0.3
PowerUp SYBR Green (2×)*	5
Template DNA (undiluted or 1:10 dilution)	2
dH2O	2.4