Figure 7.
Effect of miR-92b-3p mimic on apoptosis and luciferase reporter gene expression induced by H/R. (a) Representative pictures of Hoechst-positive nerve cells (×200). (b) Statistical results of Hoechst-positive nerve cells. (c) Flow cells of nerve cells. (d) Statistical results of flow cytometry of nerve cells. (e) Schematic of the SV40-Luci-NOX4 3′UTR-PolyA plasmid. (f) Statistics of luciferase activity in the different groups. Control: blank control group; H/R: hypoxic/reoxygenation group; + mimic: hypoxic/reoxygenation+miR-92b-3p mimic (100 nmol/l) group; +NC mimic: hypoxic/reoxygenation+negative control (100 nmol/l) mimic group; ∗P < 0.05; ∗∗P < 0.01, n = 6.