. 2022 Mar 15;15(3):131–144.

Table 2.

Reverse transcription real time polymerase chain reaction (RT-qPCR) primer sequences

Gene	Primer 5’-3’	Amplicon size	Annealing
MGMT^*	F-GCTGAATGCCTATTTCCACCA	124 bp	62°C
MGMT^*	R-CACAACCTTCAGCAGCTTCCA	124 bp	62°C
MLH1^**	F-CAACAAGTCTGACCTCGTCTTC	80 bp	60°C
MLH1^**	R-CCGGGAATCTGTACGAACCAT	80 bp	60°C
GAPDH^***	F-CCACTCCTCCACCTTTGAC	154 pb	60°C
GAPDH^***	R-ACCCTGTTGCTGTAGCCA	154 pb	60°C

Ref [52];

^**

Gene ID: 4292; GenBank NM_001167619;

^***

Gene ID: 14433; GenBank NM_008084.2.